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TaqMan® Mutation Detection Assays

Information

運用competitive allele-specific TaqMan PCR (castPCR™)的技術,偵測單點突變,偵測原理是以專一性引子偵測突變位點,以MGB blocker佔住wild type與突變點對應的allele可測得0.1%以上的mutation rate現有已設計好的778assay,含括46個癌症相關基因例如:KRAS BRAFKITJAK2 EGFR

針對不同的癌症相關基因,提供在該基因已知無mutation存在區域的TaqMan reference assay,以取得精準的突變率,同時亦可選用 (Internal Positive Control) IPC,以了解無突變存在是真實情形抑或是PCR敗。

樣本需求30 ng gDNA

Support

TaqMan® Mutation Detection IPC Reagent Kit內含模板、一對專一性引子以及一段5’端標記VIC ®螢光3’端標記TAMRATM螢光作為Quencher的探針

Category

Product Family

Description

Pack Size

Gene Expression

TaqMan® Fast Advanced Master Mix

Highest performing master mix for gene expression applications

5ml pack

 

TaqMan® Universal Master Mix II

Ideal for customers working with multiple applications including SNP and CNV assays

5ml pack

 

TaqMan® Gene Expression Master Mix

Precise quantification and sensitive detection down to one copy of target

5ml pack

 

TaqMan® Universal PCR Master Mix

Ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications

5ml pack

 

TaqMan® Fast Universal PCR Master Mix

Delivers results in 40 minutes for 40 cycles of PCR using the Applied Biosystems® 7900HT and Fast 7500 Fast Real-Time PCR Systems

5-pack

 

Platinum® Quantitative PCR SuperMix-UDG

Optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.

500 reactions

 

EXPRESS qPCR Supermix Universal

Proven Invitrogen enzymes specifically tailored to deliver superior results with probe-based qPCR assays

500 reactions

Genotyping

TaqMan® GTXpress™ Master Mix

Delivers accurate genotyping results with robust performance in less than 50 min

10ml pack

 

TaqMan® Genotyping Master Mix

Specifically formulated for detection of SNPs and insertions/deletions

10ml pack

 

TaqMan® Sample-to-SNP™ Kit

Go from biological sample to genotyping results in less than one hour

5ml sample prep and 1 ml PCR

Pre-Amp

TaqMan® PreAmp Master Mix

Preamplifies limited cDNA without introducing amplification bias to the sample

40 reactions




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