客製化細胞模型

我們的客製化細胞服務提供您哺乳類細胞株(mammalian immortalized cell lines)、初代細胞株(primary cell lines)以及多功能幹細胞株(pluripotent stem cell lines)等細胞株的基因改造，包括基因剔除(gene knockout)、基因標記(gene tag)、核苷酸取代(nucleotide substitution)、序列互換(sequence swapping)、序列整合(integration)以及序列取代(replacement)等。

Gene Knockout Service

我們與您一同攜手擬定最佳的策略，設計出最符合您實驗需求的細胞模型，經由下列方法進行基因剔除：

• Introducing mutations to disrupt the coding frame
• Inserting an exogenous cassette to prevent gene expression
• Deleting coding gene sequences

 

Gene Modification Service

在科學研究上，基因改造細胞的應用性幾乎是無限的。我們針對您的計畫需求擬定策略，設計最適合您的細胞模型，包括：

• Gene tagging to enable simple, reliable tracking and monitoring
• Nucleotide substitution to decipher molecular mechanisms
• Promoter or regulatory sequence swapping or deletion to provide a powerful tool to decipher in vivo regulatory mechanisms
• Mutation or DNA fragment introduction
• DNA fragment replacement
• DNA fragment deletion

 

Quick Knockin™ into Permissive Loci HPRT, Rosa26 or AAVS1

Knockin模型日漸複雜，genOway為齧齒類和細胞模型開發了Quick Knockin技術。利用基因位點Rosa26、HPRT以及AAVS1，有效降低模型開發的時間和花費，提供給您：

• Stable expression pattern
• Known copy number
• No position effect variegation
• No transgene silencing
• No deregulation of neighboring genes

 

Phenotyping Service

我們提供您全方位的細胞表型測試，確保基因改造細胞的功能性特徵，並經由下列方法與未改造的細胞進行測試比較：

• Determination of the expression of your target gene on mRNA level by RT-PCR and/or RT-qPCR
• Target protein profiling by microscopy, cytometry and biochemistry
• Functional assessment of the genetic modification at protein level by reliable standard in vitro assays (cell health assays, organelle and macrostructure labeling, migration, invasion and adhesion assays, metabolism and morphometry, secretion assays, and protein translocation)

 

Project Assessment & Development Process